Composite
Part:BBa_K2834005:Design
Designed by: Viana Isabel Pérez Domínguez Group: iGEM18_Tec-Chihuahua (2018-10-07)
Expressible defensin 1 antimicrobial peptide from Apis mellifera
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 233
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 86
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
For the correct expression of this peptide, a chassis with the T7 RNA polymerase gene such as E. coli BL21 (DE3) is needed. An IPTG-inducible promoter was considered for the composite in order to control the expression levels.
Source
This part was designed using basic ones: T7 promoter + RBS (BBa_K525998) + pelB leader sequence (BBa_J32015) + Defensin 1 (BBa_K1104301) + 6x His-Tag (BBa_K2834002) + T1 terminator from E. coli rrnB (BBa_B0010).